You are viewing the site in preview mode

Skip to main content

Table 4 The taxonomic classification of core OTUs (> 90% of all samples) based on RDP classification (v.10). The results of repeated measures ANOVA of the relative abundance of each OTU is also included. F statistics are included with degrees of freedom (Fdf, dfDen) where dfDen is the denominator df based on Kenward-Roger first order approximations with Kacker-Harville correction. * p < 0.05, ** p < 0.01, *** p < 0.001. Where significant, partial η2 effect sizes are presented parenthetically

From: Recovery and resiliency of skin microbial communities on the southern leopard frog (Lithobates sphenocephalus) following two biotic disturbances

Phylum (Order) Family (Genus) OTU # Treatment Time Treatment x Time
Actinobacteria
 Actinomycetales Corynebacteriaceae (Corynebacterium) 76 F3,23.2 = 2.364 F5,101.7 = 13.511***(0.354) F15,100.9 = 1.305
Dermacoccaceae (Dermacoccus) 28 F3,22.3 = 1.183 F5,100.0 = 29.749***(0.547) F15,99.3 = 2.643**(0.244)
Micrococcaceae (Micrococcus) 31i F3,23.4 = 1.482 F5,106.2 = 30.343***(0.552) F15,105.8 = 1.459
Micrococcaceae (Nesterenkonia) 2a
24
F3,18.9 = 0.454
F3,18.9 = 0.409
F5,103.6 = 23.845***(0.492)
F5,100.0 = 23.353***(0.487)
F15,103.2 = 3.206***(0.281)
F15,99.6 = 3.253***(0.284)
Propionibacteriaceae (Propionibacterium) 9 F3,18.9 = 1.270 F5,99.2 = 16.822***(0.406) F15,98.1 = 3.849***(0.319)
Bacteriodetes
 Flavobacteriales Flavobacteriaceae (Flavobacterium) 15
81
F3,22.1 = 0.478
F3,17.6 = 0.396
F5,99.5 = 9.886***(0.288)
F5,89.2 = 12.982***(0.345)
F15,99.0 = 1.387
F15,88.6 = 0.877
Firmicutes
 Bacillales Bacillales incertae sedis (Caldalkalibacillus) 3a
26a
102
F3,22.1 = 1.657
F3,22.3 = 2.954
F3,19.7 = 0.182
F5,107.6 = 20.691***(0.456)
F5,109.3 = 19.829***(0.446)
F5,89.9 = 9.516***(0.279)
F15,107.2 = 2.807***(0.255)
F15,108.8 = 2.068*(0.201)
F15,88.7 = 2.069*(0.201)
Bacillaceae (Bacillus) 29 F3,21.4 = 1.246 F5,10,442 = 16.592***(0.402) F15,103.9 = 1.998*(0.196)
Bacillaceae (Geobacillus) 100 F3,20.2 = 2.921 F5,93.3 = 13.386***(0.352) F15,92.6 = 1.267
Proteobacteria
 Burkholderiales Alcaligenaceae (Bordetella) 1i F3,22.2 = 0.304 F5,101.9 = 17.454***(0.415) F15,101.6 = 0.889
Comamonadaceae (Delftia) 16i F3,25.0 = 0.793 F5,107.8 = 14.084***(0.364) F15,107.4 = 2.077*(0.202)
Comamonadaceae (Comamonas) 18i F3,23.2 = 1.510 F5,103.1 = 7.340***(0.229) F15,102.3 = 1.731
Comamonadaceae (unclassified) 41 F3,22.6 = 1.869 F5,103.7 = 9.564***(0.281) F15,103.5 = 1.111
 Pseudomonadales Moraxellaceae (Acinetobacter) 5i
20e
F3,22.1 = 0.498
F3,24.7 = 0.724
F5,105.7 = 11.316***(0.315)
F5,100.7 = 11.135***(0.311)
F15,105.3 = 1.139
F15,100.5 = 1.202
Moraxellaceae (Enhydrobacter) 25 F3,22.6 = 5.52**§(0.118) F5,109.2 = 12.433***(0.335) F15,107.7 = 1.397
Pseudomonadaceae (Pseudomonas) 10i F3,21.6 = 0.634 F5,97.7 = 7.911***(0.243) F15,96.8 = 0.931
 Oceanospirillales Halomonadaceae (Halomonas) 17a F3,23.3 = 1.485 F5,108.9 = 26.467***(0.518) F15,108.5 = 2.392**(0.225)
 Enterobacteriales Enterobacteriaceae (Cronobacter) 21i F3,21.9 = 0.623 F5,102.2 = 12.679***(0.340) F15,101.8 = 1.091
 Methylophilales Methylophilaceae (Methylophilus) 49 F3,20.7 = 2.291 F5,94.0 = 5.107***(0.172) F15,93.4 = 1.047
 Sphingomonadales Sphingomonadaceae (Sphingomonas) 52 F3,22.3 = 1.350 F5,99.8 = 18.892***(0.434) F15,99.3 = 1.227
  1. a = OTUs present in all frogs at all time points
  2. i = OTUs with 399% sequence similarity for Bd-inhibiting bacteria (Woodhams et al. 2015)
  3. e = OTUs with 399% sequence similarity for Bd-enhancing bacteria (Woodhams et al. 2015)
  4. §=Treatment effect - Tukey HSD indicates Bd (A), BB (AB), N (B), Btk (B) where A > B